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Image Search Results
Journal: Cellular and Molecular Immunology
Article Title: MicroRNA-663 induces immune dysregulation by inhibiting TGF-β1 production in bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus
doi: 10.1038/cmi.2018.1
Figure Lengend Snippet: MiR-663 downregulates the immunoregulatory effects of BMSCs in vitro. (a–d) Percentages of Th17 cells (IL-17 A+/CD4+ T cells, (a), Treg cells (CD25+Foxp3+/CD4+ T cells, (b), Tfh cells (CXCR5++PD-1++/CD4+ T cells, (c), and plasma cells (CD19-CD138+ cells, (d) after co-culturing of pre-stimulated naïve T cells with miR-663-related vector-transfected BMSCs. (e) The percentage of proliferated Foxp3+ cells in sorted CD4+CD25+ T cells after co-culture with different BMSCs. (f) The absolute number of Foxp3+CD4+CD25+ T cells increased in the presence of miR-663-I BMSCs. (g and h) The effects of miR-663-related BMSCs on Treg cell differentiation and the conversion of Treg cells to Tfh cells. (i) The ratio of Tfh (CXCR5++PD-1++/CD4+ T cells) to Treg (CD25+Foxp3+/CD4+ T cells) after co-culturing of pre-stimulated naïve T cells with miR-663-related BMSCs. All data represent the mean±SEM. n=6, *P<0.05, **P<0.01, ***P<0.001.
Article Snippet: FCM analysis The following antibodies were applied in this study: fluorescein isothiocyanate (FITC)-conjugated anti-human CD4, HLA-DR (BD Biosciences), CD34 and CD44 (BD eBioscience), phycoerythrin (PE)-conjugated
Techniques: In Vitro, Plasmid Preparation, Transfection, Co-Culture Assay, Cell Differentiation